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Next-generation sequencers — selection and configuration
NGS platforms determine nucleic acid sequences in parallel. Platform selection depends on the workflow, including targeted panels, microbial genomes, transcriptomics and other research applications.
Select equipment and request a quotation
State your method, workload and required options to prepare a comparable quotation.
Next-generation sequencing systems
Compare reads per run, read length, data output and library preparation requirements. Consider consumables, data storage and bioinformatics separately. Research or diagnostic intended use is defined by the documentation for each system.
Compare three sequencers
| Model | XLEAP-SBS flow cells and output |
|---|---|
| Illumina MiSeq i100 | 5M / 25M; up to 7.5 Gb at 2 × 150 bp; 2 × 500 bp on 25M only |
| Illumina MiSeq i100 Plus | 5M / 25M / 50M / 100M; up to 30 Gb at 2 × 150 bp; 2 × 500 bp on 25M only |
| Illumina NextSeq 2000 | P1 / P2 / P3 / P4; up to 540 Gb at 2 × 150 bp; 2 × 300 bp on P1/P2 only |
All three models are Research Use Only. Compare equivalent read modes and reagent kits: maximum data output and longest reads use different configurations. Instrument pricing and consumables are quoted separately.
Manufacturer information and documents
Check the version, supply region and document revision. A series page may describe options that are not included in the specific configuration.
Configuration, pricing and procurement
For a quotation, specify the required configuration, number of instruments, installation city and services needed. Agree options, consumables, commissioning and staff familiarisation separately. Documentation, delivery times and warranty terms are specified in the quotation and contract. See the model page for its current price or quotation request option.
Questions before selection and procurement
Which models are listed?
Illumina MiSeq i100 · Illumina MiSeq i100 Plus · Illumina NextSeq 2000.
How does MiSeq i100 Plus differ from MiSeq i100?
MiSeq i100 uses 5M and 25M flow cells. Plus also supports 50M and 100M. The 2 × 500 bp mode requires the 25M / 1000-cycle kit and software v1.1.0 or later; it is not available on 100M.
What does 540 Gb mean for NextSeq 2000?
This is the output of one P4 XLEAP-SBS run at 2 × 150 bp, taking approximately 44 hours. Only P1 and P2 support 2 × 300 bp, with different output and run time. Manufacturer specifications use PhiX; actual results depend on the library.
What should a quotation request include?
Specify the exact instrument, flow cell, read length, number of runs, reagents and library preparation kits. Agree analysis, data storage, installation, training and service. All listed instruments are RUO, not for diagnostic procedures.